ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (5): 728-737.doi: 10.11843/j.issn.0366-6964.2015.05.007

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Molecular Cloning and Biological Characterization Analysis of Toll-like Receptors 1-10 in Maiwa Yak

LIN Bao-shan,LAN Dao-liang*,CHEN Ya-bing,HUANG Cai,FU Mei,LI Jie,LI Jian*   

  1. (College of Life Science and Technology,Southwest University for Nationalities / College of Tibetan Plateau Research,Southwest University for Nationalities,Chengdu 610041,China)
  • Received:2014-08-01 Online:2015-05-23 Published:2015-05-23

Abstract:

In this study,the coding region of Maiwa yak toll-like receptors 1-10 genes were cloned,and then the bioinformatics tool was used to analyze the characteristics of TLRs sequences,and real-time PCR was used to detect the expression pattern of TLRs.The results showed that Maiwa yak TLR genes exhibited high homologies at the nucleotide and the amino acid levels with other species.Phylogenetic relationships showed that Maiwa yak had a nearest relationship with cattle and sheep,Maiwa yak was clustered into a branch with human,horse and mouse.It was noteworthy that TLR1,TLR6 first clustered into a small branch,then gathered with TLR10 for a closer one,then TLR1,2,6,10 and TLR7,8,9 were gathered in the 2 individual branches,respectively.The other members of TLRs became respective one.Real-time PCR results showed that TLRs were expressed in all tissues of yak,but different members had different expression patterns.TLR2,TLR4 and TLR6 had the highest expression in the spleen,followed with ovary,small intestine,kidney,liver.TLR1,TLR5,TLR7,TLR8,TLR9 and TLR10 had the highest expression in kidney,higher expression in liver,kidney,spleen.The study provide useful information for further study in immune molecular mechanisms and disease resistance breeding of yaks.

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